Alexandru Valentin ZĂGREAN, Gabriela ȘOVĂREL
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lignocellulosic substrate, mycelium, Pleurotus ostreatus, wild strain.
Specimens of Pleurotus ostreatus mushrooms from spontaneous mycobiota were collected from different regions of Romania, in order to obtain mycelial isolates, preserve and use them for the development of the cultivated assortment. The mycelia of the PoBr and Po2T strains, obtained from a first collection series, were clonally multiplied and cultured in vitro to highlight the morphophysiological and cultural characteristics of the colonies, compared to two commercial strains of P. ostreatus. The wild isolates have developed on three variants of agarized media – with potato extract (PDA), with malt extract (MEA) and with compost extract (MECA) – viable, robust mycelial colonies, with morphology similar to that of commercial strains but also with their own peculiarities. The growth rate of wild mycelia was lower than in commercial ones. A similar behavior of the new isolates was highlighted during the preparation of the seeding mycelium/spawn grown on wheat grains and, subsequently, during the colonization period, on the lignocellulosic substrate prepared in two variants for the fruiting tests.
Cite this paper: Zăgrean, A.V., Șovărel, G. (2025). EVALUATION OF WILD STRAINS OF PLEUROTUS OSTREATUS ISOLATED FROM SPONTANEOUS MYCOBIOTA BY MYCELIUM GROWTH CHARACTERISTICS IN VITRO. Current Trends in Natural Sciences, 14(28), 98-105. https://doi.org/10.47068/ctns.2025.v14i28.009
