Hasan Pinar, Aydin Uzun
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Wild pear, in vitro, sterilisation
Wild pear(Pyrus elaeagrifolia) is one of the rootstocks used in pear cultivation. It is one of our most important genetic resources, especially because it is resistant to drought conditions and iron chlorosis. Besides its positive features, its use as a rootstock also has some disadvantages. In vitro propagation methods have gained importance in clonal propagation, especially due to the difficulties encountered in germination from seeds and problems in rooting with cuttings. With this method, mass production can be achieved in a short time. However, as in other species, contamination is the biggest obstacle in in vitro culture in pear. There is no valid protocol yet for surface sterilization processes for micropropagation of wild pear. New studies are needed for this purpose. The aim of this study was to determine the sterilization conditions suitable for the shoot tip technique to be used in in vitro propagation of some pear species. In the study, 4 different sterilization methods were applied to shoot tip explants of the wild pear genotype obtained from 6 different regions, and bacterial and fungal infection and plant development were examined in the explants. According to the results of present study, the contamination rate varied according to genotype and sterilization methods. The obtained results show that determining different protocols according to genotypes in in vitro micropropagation in important species such as wild pear (Ahlat) may affect the success of micropropagation.
Cite this paper: Pinar, H., Uzun, A. (2024). DETERMINATION OF THE EFFECT OF DIFFERENT STERILIZATION PROTOCOLS FOR IN VITRO SHOOT TIP CULTURE IN SOME WILD PEAR (P. ELAEAGRIFOLIA) GENOTYPES. Current Trends in Natural Sciences, 13(25), 123-129. https://doi.org/10.47068/ctns.2024.v13i25.015
