Merve A. Yigit, Hasan Pinar, Aydın Uzun
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Apple, Callus culture, Regeneration efficiency
Genetic transformation technology emerged as a useful tool to shorten the breeding time in plants and also allowed the improvement of the plants by only intended character without changing their other characters. To carry out gene transfer studies, the regeneration efficiency of plants must be known. In this study, in vitro regeneration efficiency was investigated in 9 genotypes in 3 different apple species; Malus domestica, Malus sieversii and Malus niedzwetzkyana. After explants shooted in the media, callus culture was applied to sterile leaves. Genotypes' shoot formation ratio was ranged from 8.33% to 75%. The genotype with the highest shoot was 'genotype 8', species of M. domestica, with a rate of 75%. Sterile leaves were taken from shoots for callus culture. The regeneration potential of genotypes ranged from 0% to 90%. While callus formation was not observed in 'genotype 4' and 'genotype 5' (M. domestica), 90% callus formation was observed in 'genotype 3' (M. domestica). With this study, it has been determined that there is a serious variation in the regeneration efficiency of different apple species and genotypes.
Cite this paper: Yigit, M.A., Pinar, H., Uzun, A. (2020). DETERMINATION OF REGENERATION EFFICIENCY IN DIFFERENT APPLE SPECIES AND VARIETIES. Current Trends in Natural Sciences, 9(18), 149-153. https://doi.org/10.47068/ctns.2020.v9i18.020
