Roxana Gabriela Cristina, Ioan Ardelean
Full Text PDF | Black Sea water, colony forming units, fluorescence, microcosm, Salmonella enterica var. Poona.
This study focuses on the determination of the viability of Salmonella enterica var. poona sub-population submerged in indoor sea water microcosms for a period of five days. The usage of bought filtered and unfiltered sea water and two different temperatures (4°C and 37°C) aimed to establish the importance of biotic and abiotic parameters of the microcosms in the dynamic of the Salmonella enterica var. poona cells. The collected probes were analysed via fluorescent microscopy with respect to total cell density and the number of dead and the cultivability capacity of the bacteria cells which was assessed by CFU determination on selective culture media (Luria Bertani and Salmonella Shigella Agar). The results obtained in this study show that in this five days period the temperature of the sea water and the presence of concurrent bacteria and protozoa are contributing significantly to the decrease of viability and multiplication capacity of Salmonella enterica var. poona that undergone a hypo-osmotic shock after the passage in the indoor microcosms. Low temperature (4°C) and protozoa predation (unfiltered sea water) seemed to be the most harmful microcosm configuration regarding bacterial survivability leading to near annihilation of the cultivability capacity at the end of the five days studied time.